hnRNP C1/C2 Polyclonal Antibody

Sizes: 50µl, 100µl

Catalogue Numbers: BS90655-50, BS90655-100

Product: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Swiss-Prot: P07910(Human) Q9Z204(Mouse) P17132(Rat)

Host: Rabbit

Reactivity: Human, Mouse, Rat, zebrafish

Applications: WB, ICC/IF, IHC, IP, , FC

All Applications: WB:1:1,000-1:5,000
ICC:1:100-1:500
IHC:1:50-1:200
FC:1:50-1:100

Background: Heterogeneous nuclear ribonucleoproteins (hnRNPs) constitute a set of polypeptides that contribute to pre-mRNA processing and transport, and also bind heterogeneous nuclear RNA (hnRNA), which are the transcripts produced by RNA polymerase II. hnRNP complexes are the major constituents of the spliceosome and, in particular, the hnRNP A1 protein is one of the major pre-mRNA/mRNA binding proteins and also one of the most abundant proteins in the nucleus. hnRNP A1 and A2/B1 regulate the processing of pre-mRNA by directly antagonizing the association of various splicing factors and by influencing the splice site selection on pre-mRNA. The majority of hnRNP proteins components are localized to the nucleus; however, some shuttle between the nucleus and the cytoplasm. Most hnRNP proteins, in-cluding hnRNP C1 and C2, contain one or more RNA binding domains and are implicated in the processing of pre-mRNA. hnRNPs F and H are largely related factors that preferentially associate with poly(rG) regions on RNA. Isoforms of these proteins are often generated by alternative processing of the pre-mRNA and by posttranslational modifications such as phosphorylation on serines and threonines and methylation of arginines.

Purification and Purity: ProA affinity purified

Storage and Stability: Store at +4°C after thawing. Aliquot store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.

Specificity: hnRNP C1/C2 polyclonal antibody detects endogenous levels of hnRNP C1/C2 protein.

Bioworld Molecular Weight: 42 kDa

Note: For research use only, not for use in diagnostic procedure.

Extra Notes: Western blot analysis of hnRNP C1+C2 on different lysates using anti-hnRNP C1+C2 antibody at 1/1,000 dilution.
Positive control:
Lane 1: Hela
Lane 2: MCF-7
Lane 3: HepG2, ICC staining hnRNP C1+C2 in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

Alternative Name: hnRNP C1/C2, HNRNPC, C1, C2, Heterogeneous nuclear ribonucleoprotein C (C1/C2), Heterogeneous nuclear ribonucleoprotein C, Heterogeneous nuclear ribonucleoproteins C1/C2, HNRNP, hnRNP C1 / hnRNP C2, hnRNP C1/C2, Hnrnpc, HNRPC, HNRPC, MGC104306, MGC105117, MGC117353, MGC131677, Nuclear ribonucleoprotein particle C1 protein, Nuclear ribonucleoprotein particle C2 protein, SNRPC,

Immunogen: recombinant protein

Conjugate: Unconjugated

Modification: Unmodified