Recombinant Human PACT protein (His tag)

Size: 100μg

Catalogue Numbers: PDEH100367-100

Citations, Manuals and MSDS Available upon request.

Abbreviation: PACT

Target Synonym: Interferon-inducible double-stranded RNA-dependent protein kinase activator A; PRKRA; PKR-associated protein X

UNIProt ID: O75569

Research Areas: Epigenetics and Nuclear
signaling

Target Species: Human

Expression Host: E.coli

Fusion Tag: N-His & C-His

Accession: O75569

Background: Activates EIF2AK2/PKR in the absence of double stranded RNA (dsRNA), leading to phosphorylation of EIF2S1/EFI2-alpha and inhibition of translation and induction of apoptosis. Required for siRNA production by DICER1 and for subsequent siRNA-mediated post-transcriptional gene silencing. Does not seem to be required for processing of pre-miRNA to miRNA by DICER1.

Sequence: Met 1-Lys 313

Purity: > 95 % as determined by reducing SDS-PAGE.

Formulation: Lyophilized from sterile PBS, pH 7.4.
Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.
Please refer to the specific buffer information in the printed manual.

Reconstitution: Please refer to the printed manual for detailed information.

Endotoxin: Please contact us for more information.

Calculated MW: 34.32 kDa

Observed MW: 38 kDa

Storage: Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80℃. Reconstituted protein solution can be stored at 4-8℃ for 2-7 days. Aliquots of reconstituted samples are stable at < -20℃ for 3 months.

Shipping: This product is provided as lyophilized powder which is shipped with ice packs.

Research Use Only