Recombinant Human PPT1 Protein (His Tag)

Sizes: 10μg, 50μg

Catalogue Numbers: PKSH032848-10, PKSH032848-50

Citations, Manuals and MSDS Available upon request.

Abbreviation: PPT1

Target Synonym: Palmitoyl-protein thioesterase 1; PPT-1; Palmitoyl-protein hydrolase 1; PPT1

UNIProt ID: P50897

Research Areas: Signal Transduction; Cancer; metabolism;

Target Species: Human

Expression Host: HEK293 Cells

Fusion Tag: C-6His

Accession: P50897

Background: Palmitoyl-protein thioesterase 1(PPT-1 for short), also known as Palmitoyl-protein hydrolase 1, belongs to the palmitoyl-protein thioesterase family. It is a small glycoprotein involved in the catabolism of lipid-modified proteins during lysosomal degradation. This enzyme removes thioester-linked fatty acyl groups such as palmitate from modified cysteine residues in proteins or peptides during lysosomal degradation. Defects in PPT1 are the cause of neuronal ceroid lipofuscinosis type 1.

Sequence: Asp28-Gly306

Purity: > 90 % as determined by reducing SDS-PAGE.

Formulation: Supplied as a 0.2 μm filtered solution of 20mM Tris-HCl, 150mM NaCl, 10%Glycerol, pH7.5.
Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.
Please refer to the specific buffer information in the printed manual.

Reconstitution: Not Applicable

Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method.

Calculated MW: 32.3 kDa

Observed MW: 37 kDa

Storage: Store at < -20°C, stable for 6 months. Please minimize freeze-thaw cycles.

Shipping: This product is provided as liquid. It is shipped at frozen temperature with blue ice/gel packs. Upon receipt, store it immediately at < - 20°C.

Research Use Only