LGR5/GPR49 Polyclonal Antibody

Sizes: 50µl, 100µl

Catalogue Numbers: BS90797-50, BS90797-100

Product: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Swiss-Prot: O75473(Human) Q9Z1P4(Mouse) Unigene: 214063(Rat)

Host: Rabbit

Reactivity: Human, Mouse, Rat

Applications: WB, IP, , FC

All Applications: WB:1:1,000
FC:1:50-1:100

Background: G protein-coupled receptors (GPCRs), also designated seven transmembrane (7TM) receptors or heptahelical receptors, interact with G proteins (heterotrimeric GTPases) to synthesize intracellular second messengers, such as diacylglycerol, cyclic AMP, inositol phosphates and calcium ions. Their diverse biological functions range from vision and olfaction to neuronal and endocrine signaling and are involved in many pathological conditions. LGR5 (leucine-rich repeat-containing G-protein coupled receptor 5), also known as GPR49 or GPR67, is a 907 amino acid multi-pass membrane protein that contains 17 leucine-rich repeats and belongs to the G protein-coupled receptor family. Expressed in placenta, skeletal muscle and spinal cord, LGR5 functions as an orphan receptor that is thought to play an important role in embryonic growth control and cellular differentiation. Overexpression of LGR5 is associated with increased tumor susceptibility and malignant transformation, implicating LGR5 as a potent tumor-inducing protein.

Purification and Purity: ProA affinity purified

Storage and Stability: Store at +4°C after thawing. Aliquot store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.

Specificity: LGR5/GPR49 polyclonal antibody detects endogenous levels of LGR5/GPR49 protein.

Bioworld Molecular Weight: 100 kDa

Note: For research use only, not for use in diagnostic procedure.

Extra Notes: Western blot analysis of LGR5/GPR49 on SH-SY-5Y lysates using anti-LGR5/GPR49 antibody at 1/1,000 dilution., Flow cytometric analysis of SH-SY-5Y cells with LGR5/GPR49 antibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody.

Alternative Name: LGR5, GPR49, GPR67, FEX, G protein coupled receptor 49, G protein coupled receptor 67, g protein-coupled receptor fex, G-protein coupled receptor 49, G-protein coupled receptor 67, G-protein coupled receptor HG38, GPR 49, GPR 67, GPR49, GPR67, GRP 49, GRP49, HG 38, HG38, Leucine rich repeat containing G protein coupled receptor 5, Leucine-rich repeat-containing G-protein coupled receptor 5, LGR 5, LGR5, LGR5, MGC117008, Orphan G protein coupled receptor HG38,

Immunogen: recombinant protein

Conjugate: Unconjugated

Modification: Unmodified