JNK3 Polyclonal Antibody

Sizes: 50µl, 100µl

Catalogue Numbers: BS90754-50, BS90754-100

Product: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Swiss-Prot: P53779(Human) Q61831(Mouse) P49187(Rat)

Host: Rabbit

Reactivity: Human, Mouse, Rat

Applications: WB, ICC/IF FC

All Applications: WB:1:500
FC:1:50-1:100
ICC:1:100-1:500

Background: c-Jun N-terminal kinases (JNKs) phosphorylate and augment transcriptional activity of c-Jun. JNKs originate from three genes that yield 10 isoforms through alternative mRNA splicing, including JNK1a1,JNK1b1, JNK2a1, JNK2b1, and JNK3a1, which represent the p46 isoforms, and JNK1a2, JNK1b2, JNK2a2, JNK2b2, and JNK3b2, which represent the p54 isoforms.JNKs coordinate cell responses to stress and influence regulation of cell growth and transformation. The human JNK1 (PRKM8, SAPK1, MAPK8) gene maps to chromosome 10q11.22 and shares 83% amino acid identity with JNK2. JNK1 is necessary for normal activation and differentiation of CD4 helper T (TH) cells into TH1 and TH2 effector cells. JNK3 (MK10, MAPK10, PRKM10) is activated by pro-inflammatory cytokines and environmental stresss by phosphorylating transcription factors such as c-Jun and ATF2. This is important for AP-1 transcriptional activity regulation. JNK3 is crucial for neuronal apoptosis (stress-induced).

Purification and Purity: ProA affinity purified

Storage and Stability: Store at +4°C after thawing. Aliquot store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.

Specificity: JNK3 polyclonal antibody detects endogenous levels of JNK3 protein.

Bioworld Molecular Weight: 53 kDa

Note: For research use only, not for use in diagnostic procedure.

Extra Notes: Western blot analysis of JNK3 on 293T cells lysates using anti-JNK3 antibody at 1/1,000 dilution., ICC staining JNK3 in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

Alternative Name: MAP kinase 10, MAPK 10, SAPK1b, MAPK10, c Jun kinase 3, c-Jun N-terminal kinase 3, cJun N terminal kinase 3, FLJ12099, FLJ33785, JNK3 alpha protein kinase, JNK3, JNK3A, MAP kinase 10, MAP kinase, MAP kinase p49 3F12, MAPK 10, Mapk10, MGC50974, mitogen activated protein kinase 10, Mitogen-activated protein kinase 10, MK10, p493F12, p54bSAPK, PRKM10, protein kinase mitogen activated 10, SAPK1b, Stress activated protein kinase 1b, stress activated protein kinase beta, Stress activated protein kinase JNK3, Stress-activated protein kinase JNK3,

Immunogen: recombinant protein

Conjugate: Unconjugated

Modification: Unmodified